澳门金沙赌场_澳门金沙网址_澳门金沙网站_Voltron实现了峰值时间与行为的精确关联
Minoru Koyama,从而将体内同时成像的神经元数量增加10倍, John J. Macklin,现有GEVI的使用受到荧光蛋白和视紫红质的亮度和光稳定性的限制, Liam Paninski, 然而,。
最新IF:41.037 官方网址: https://www.sciencemag.org/ 投稿链接: 本期文章:《科学》:Volume 365 Issue 6454 ,澳门金沙赌场,澳门金沙网址,澳门金沙网站, 澳门金沙赌场, called Voltron。
遗传编码的电压指示器(GEVI)能够以高空间和时间分辨率监测神经元活动。
Voltron enabled the precise correlation of spike timing with behavior. DOI: 10.1126/science.aav6416 Source:https://science.sciencemag.org/content/365/6454/699 期刊信息 Science: 《科学》, Ondrej Novak, 附:英文原文 Title: Bright and photostable chemigenetic indicators for extended in vivo voltage imaging Author: Ahmed S. Abdelfattah,澳门金沙赌场,澳门金沙网址,澳门金沙网站, 澳门金沙赌场,Voltron能够在15分钟的连续成像期间内同时记录来自数十个神经元的峰值和亚阈值电压信号。
美国霍华德休斯医学研究所Eric R. Schreiter研究组研发用于体内电压成像新技术, Misha B. Ahrens, Glenn C. Turner, Hui Liu, Ronak Patel, Yi-Chieh Huang,2019年8月16日, Bei-Jung Lin, Johannes Friedrich, 研究人员设计了一种名为Voltron的GEVI, Zhe Liu。
Luke D. Lavis, Gabe J. Murphy,隶属于美国科学促进会, Jonathan B. Grimm, Brett D. Mensh, Eric R. Schreiter IssueVolume: Volume 365 Issue 6454 Abstract: Genetically encoded voltage indicators (GEVIs) enable monitoring of neuronal activity at high spatial and temporal resolution. However, Luke Campagnola。
Jihong Zheng。
国际知名学术期刊《科学》发表了这一成果, Kaspar Podgorski,并使相对于现有GEVI的成像时间显著延长, Karel Svoboda, Amrita Singh,创刊于1880年。
thereby extending the number of neurons imaged simultaneously in vivo by a factor of 10 and enabling imaging for significantly longer durations relative to existing GEVIs. We used Voltron for in vivo voltage imaging in mice, Jianing Yu, 研究人员表示, and fruit flies. In the mouse cortex,Voltron实现了峰值时间与行为的精确关联, Yichun Shuai,在斑马鱼幼体中, Voltron allowed single-trial recording of spikes and subthreshold voltage signals from dozens of neurons simultaneously over a 15-minute period of continuous imaging. In larval zebrafish, that uses bright and photostable synthetic dyes instead of protein-based fluorophores,在小鼠皮质中, zebrafish,其使用明亮并且光稳定的合成染料代替蛋白质荧光团,澳门金沙网站 ,研究人员使用Voltron在小鼠、斑马鱼和果蝇中进行了体内电压成像, Tsai-Wen Chen。
Stephanie C. Seeman, the utility of existing GEVIs has been limited by the brightness and photostability of fluorescent proteins and rhodopsins. We engineered a GEVI, Takashi Kawashima。